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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
431804 | 5 plates | £209 | ||||
431815 | 10 Plates | £401 | ||||
431816 | 20 Plates | £642 |
Description
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend's ELISA Max⢠Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!
BioLegend's ELISA Max⢠Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max⢠Set Standard and ELISA Max⢠Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
Human IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Product Details
Kit Contents
- Kit Contents
-
- Human IL-2 ELISA MAX™ Capture Antibody (200X)
- Human IL-2 ELISA MAX™ Detection Antibody (200X)
- Human IL-2 Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Assay Diluent A (5X)
Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution F mentioned on the TDS and the current manual.Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.
Product Details
- Verified Reactivity
- Human
- Application
- ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Application Notes
The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.
- Application References
(PubMed link indicates BioLegend citation)
- Product Citations
-
- Yang L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
- Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
- Tello‐Lafoz M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16370-w. PubMed
- Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
- Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
- Stürner K, et al. 2014. J Immunol. 193:4439. PubMed
- Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
- Waldrip ZJ, et al. 2021. J Biol Chem. 297:101209. PubMed
- Gigliotti CL, et al. 2022. Front Immunol. 13:915963. PubMed
- Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
- Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
- Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
- Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
- Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
- Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
- Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
- Feng Y, et al. 2022. Life (Basel). 12:. PubMed
- Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
- Lee C, et al. 2020. Biomaterials. 262:120338. PubMed
- Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
- Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
- Sensitivity
- 4 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2 SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2 HPO4 , 0.2 g KH2 PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Sources
- T cells
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Products
Description | Clone | Applications |
---|
Pathways
- Cancer Immunoediting
- Hematopoiesis from Multipotent Stem Cell
- Hematopoiesis from Pluripotent Stem Cell
- ICos-ICosL Pathway in T-Helper Cell
- IL-2 Pathway
- Immunologic Networks 2011
- MDSC-induced Immune Suppression
- NFAT Signaling and Lymphocyte Interactions
- Regulatory T Cells
- TH2 Pathway (Humoral Immune Response)
Pages
- Quality Control
- ELISA Kits and Sets
Related FAQs
- Do the ELISA MAX⢠Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
Certificate of Analysis
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For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
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